Thin Layer Chromatography- Principle, Applications and Procedure
Thin Layer Chromatography(TLC) is a separation technique used to separate the components of non-volatile mixtures. TLC is significantly a better separation process than Paper chromatography. TLC was first reported to be used in 1938 in Izmailov and Schreiber. Principle of Thin Layer Chromatography Thin Layer Chromatography is a separation process based on the principle of adsorption in which the components of sample separate based on their affinity towards the stationary and mobile phase where the stationary phase is a thin layer of adsorbent material such as Aluminium oxide, Cellulose, or Silica gel while the mobile phase is the solvent. The separation occurs in a way that the components of higher affinity in a mixture move faster than those which have less affinity towards the stationary phase. When the solvent reaches the solvent front the components are placed at different Rf values and thus the separation is achieved. This procedure of […]